Ultrasonic cleansings of staphylococcal infected skin wound

An experiment was done in guinea pigs to evaluate the efficacy of therapeutic ultrasound, using the under water technique, for cleansing of infected wounds. A. triangular wound in the dorsal aspect of 75 guinea pigs were done under general anaesthesia. The wounds in 60 guinea pigs were infected by placing one-milliliter of the diluted culture; containing 1x10[5] staphylococcus aureus, on its surface, After 24 hours, animals with infected wounds were randomly assigned to one of the four groups. The wounds in group [I] were daily cleaned with pulsed ultrasound 1.5 w/cm for five minutes, using the underwater technique, while in group [II] wounds were subjected to the same therapy as in group [I], but with mock-ultrasonic apparatus. The wounds in group [III] were daily subjected to the conventional method of cleansing using sterile gauze and polyvinyl povidone iodine [Betadine] solution, while the wounds in group [IV] were received no treatment. The wounds of the remaining 15 guinea pigs were not infected and acted as controls for any added infection [Group [V]] It was found that therapeutic ultrasound by the under water technique removed bacteria from experimental wounds, more efficiently than conventional procedures, and therapy reduces the likelihood of sepsis. In addition, it facilitate the process of wound healing. This effective, safe, simple and painless technique should be considered for treatment of infected wounds

Assessment of candida albicans growth level on different acrylic denture base materials, vacuum and non vacuum mixed

Candida species and microbial plaque on the tissue fitting surface of a maxillary complete denture and the palatal mucosa are the chief etiologic agent in the development and maintenance of denture stomatitis [Santarpia et al., 1988]. Denture stomatitis is a multifactorial condition associated with the wearing of a removable prosthesis. Predisposing factors include trauma [Bastiaan, 1976], candida infection [Budtz-Jorgensen and Bertram, 1970] [Renner et al., 1979] bacterial infection [Van Reenen, 1973] [Budtz-Jorgensen et al., 1983] and denture wearing habits [Etting, 1975] Candida albicans organism is part of the normal oral flora of healthy dentate adults [Young et al., 1951] as well as edentulous persons [Budtz-Jorgensen et al., 1975]. The change of candida albicans from commensalism to a pathogen in denture wearers is most probably due to alteration in the oral environment of the host rather than the alteration of the yeast itself [Young et al., 1951]The chief method of prevention and treatment of denture stomatitis is aimed at the : Ielimination of yeast and bacterial species from the denture tissue-fitting surface and denture supporting mucosa by means of an effective denture and oral hygiene cleansing regimen[Augsbruger and Elahi 1982] [Palenikand Miller, 1984]. However, a completely effective denture hygiene program for the treatment of denture stomatitis has not yet been developed [Santarpia et al., 1988]. This study was conducted to determine the level of candida albicans growth on: 1- Heat polymerized resin and auto polymerized resin bases non vacuum mixed 2-Heat polymerized resin and autopolymerized resin bases vacuum mixed 3- Trial VLC resin base

Bacterial translocation in burn injury

This experimental study was carried on 20 guinea pigs with burn injury involving 40% of their body surface areas. Animals were classified into four groups, five animals in each: Group [I]: with thermal injury perse, Group [II]: Burn wound was immediately contaminated with pathogenic staphylococci and Pseudomonas pyocyaneus, Group [III]: intestinal decontamination was achieved by giving oral combination of penicillin, Streptomycin and Metronidazol and Group [IV]: Intestinal decontamination was achieved and then animals were orally contaminated with E. coli of known serotype [078 K 80 B-] obtained from a case of haemorrhagic gastroenteritis in Mansoura University Hospital. In all groups animals were killed 48 hours after induction of burn injury. The study showed that bacterial translocation occurred maximammly [100%] in group [IV], which were orally contaminated with E. coli. The same species of E. coli was harvested from the mesentric lymph nodes. In group [III] in which the intestine was decontaminate, only 20% showed bacterial translocation and at the same time the histopathological changes were minimal where there was no mucosal erosion or disintegration of lamina propria

record on L.interogans serovar canicola in human and dogs in Dakahlia governorate

In addition to rats, dogs could be the source and reservoir for human leptospirosis both in rural and urban localities [Helsey et al. 1988]. L. canicola was reported to be responsible for human leptospirosis up to 12% of recorded cases [Lecour et al. 1989]. Leptospirosis as a clinical diagnosis is not well known by the physicians, the initial diagnosis is usually wrong because the sporadic occurance of the disease, diagnosis on admission was correct in only 18% of cases [Ziegier 1987] Various organ manifestations could be presented, febrile, toxi-infectious syndrome, liver, renal, meningeal [Kunchev et al. 1988]. This work was done to estimate the prevalence of the leptospiral infection in our locality and the prevalent causative serovar

Seroprevalence of Leptospira icterohemorhagia in the field rats and domestic animals

This work was done on 1433 serum sample[470 rats, 353 cows, 372, buffalos, 161 sheeps and 77 goats]collected from 3 different localities in Dakahlia Governorate, and examined for the seroprevalence of Leptospira icterohaemrhagia by the complement fixation test. The result of the study indicate that the leptospira icterohemorhagia is carried by[10.4%] of rats collected, which is highly significant compared to the carrier rate of cows [4.8%], buffalos [3.76%] sheep [2.48%] and goats [0.05]. There was no significant difference in the carrier rate in rats and domestic animals as regard the localities from which the animals collected

Effect of starvation and water depletion on the structure and function of the immunological system of albino rats

48 albino rats, 200-250 gm. body weight comprised the material for the present work. They were classified into 2 groups. The1st group was deprived from food while the 2nd groub was deprived from water as well. A third group of 6 animals used as control. The animals within the 1 st and 2nd groups were subdivided into 4 subgroups and used to study the effect od atarvation and stravation aasociated with dehydration for 3, 6, 9 and 12 days respectively on the structure and the function of the immunological system. It was found that atrophic changes inorm of lymphocytic epletion with absence of germinal centres occur in spleen, lymph nodes and tonsils after the first week. The atrophic changes were more marked in the group of starvation with dehydration. Also, efinite thymic involution was demonstrated from the start of the experiment in both groups. On the other hand relative increase in the gamma globulin occurred. At the same time decreased ymphocytic transformation reflecting an impairment of cell mediated immunity was demonstrated in the present study. The impact of these findings on existing public health and vaccination rograms are of importance and must be taken in consideration